Vol. 117 No. 2 (2023)
Research Papers

Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles

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Supplementary Material
  • Sara B.H. Awaly,
  • Neama H. Osman,
  • Hend M. Farag,
  • Ibrahim H. Yacoub,
  • Mohamed Mahmoud-Aly,
  • Nagwa I. Elarabi Elarabi,
  • Dalia S. Ahmed
Sara B.H. Awaly
Genetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Neama H. Osman
Genetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Hend M. Farag
Agricultural Botany Department, Faculty of Agriculture, Cairo University; Giza, 12613, Egypt
Ibrahim H. Yacoub
Agronomy Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Mohamed Mahmoud-Aly
Plant Physiology Division, Department of Agricultural Botany, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Nagwa I. Elarabi Elarabi
Genetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
Dalia S. Ahmed
Genetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
DOI: https://doi.org/10.36253/jaeid-14716

Published 2023-12-31

Keywords

  • Durum wheat,
  • Genetic diversity,
  • Salt tolerance,
  • SCoT markers,
  • Silver nanoparticles,
  • SRAP markers
    • ...More
    Less

How to Cite

Awaly, S. B., Osman, N. H., Farag, H. M., Yacoub, I. H., Mahmoud-Aly, M., Elarabi, N. I. E., & Ahmed, D. S. (2023). Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles. Journal of Agriculture and Environment for International Development (JAEID), 117(2), 161–184. https://doi.org/10.36253/jaeid-14716

Copyright (c) 2023 Sara B.H. Awaly, Neama H. Osman, Hend M. Farag, Ibrahim H. Yacoub, Mohamed Mahmoud-Aly, Nagwa I. Elarabi Elarabi, Dalia S. Ahmed

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Durum wheat is one of the most critical cereal crops in widespread cultivation and has high economic value worldwide. This study evaluated the effects of silver nanoparticles (AgNP) on durum wheat's (Triticumturgidum var. durum) ability to tolerate salinity. Seeds were soaked with 0, 10, and 20 mg/l AgNPs for 20 hours. Afterward, seedlings were transplanted into the greenhouse, where their growth continued. Plant weight, fresh weight, dry weight, sodium, potassium, and chloride ion contents were measured. According to the findings, AgNPs dramatically enhanced plant fresh- and dry-weight and the ability of plants' salinity tolerance. Likewise, the impact of AgNPs on the higher plants was significant at P ≤ 0.05. Thirty-seven start codon-targeted (SCoT) primers and forty-two sequences related amplified polymorphism (SRAP) primers were employed to assess the genetic influence of AgNPs on wheat cultivars. The SCoT and SRAP analyses showed that 226 out of 393 and 241 out of 477 markers, respectively, were detected as polymorphic markers (57.50 % and 50.52 %) among the tested wheat cultivars. In addition, the polymorphism information content (PIC), marker index (MI), effective multiplex ratio (EMR), and resolving power (RP) parameters were computed to assess the effectiveness of the markers. Overall, this study demonstrates a prospective strategy for salt tolerance and replies to queries regarding the relationship between traditional agricultural methodology and the use of nanoparticles. Additionally, it dramatically helps achieve the objective of sustainable improvement for raising crop productivity through immensely safer and greener accessibility.

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