Vol. 118 No. 2 (2024)
Research Papers

Evaluation of mycorrhizae transplantation from Panicum maximum as biofertilizer for paddy cultivation in Sri Lanka

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  • Chathurika Wanninayake,
  • Mekhala Chandrasekara,
  • Sanath Hettiarachi,
  • Wasantha Malaviarachchi,
  • Lorenzo Vergani,
  • Sara Borin
Chathurika Wanninayake
Department of Biological Sciences, Faculty of Applied Sciences, Rajarata University of Sri Lanka, Mihintale, Sri Lanka
Bio
Mekhala Chandrasekara
Department of Biological Sciences, Faculty of Applied Sciences, Rajarata University of Sri Lanka, Mihintale, Sri Lanka
Sanath Hettiarachi
Department of Biological Sciences, Faculty of Applied Sciences, Rajarata University of Sri Lanka, Mihintale, Sri Lanka
Wasantha Malaviarachchi
Field Crop Research and Development Institute, Mahailluppallama, Sri Lanka
Lorenzo Vergani
Department of Food, Environmental and Nutritional Sciences, University of Milan, Milano, Italy
Sara Borin
Department of Food, Environmental and Nutritional Sciences, University of Milan, Milano, Italy
DOI: https://doi.org/10.36253/jaeid-16213

Published 2024-12-29

Keywords

  • Arbuscular mycorrhizal fungi,
  • sustainable agriculture,
  • rice,
  • microbiome transplantation

How to Cite

Wanninayake, C., Chandrasekara, M., Hettiarachi, S., Malaviarachchi, W., Vergani, L., & Borin, S. (2024). Evaluation of mycorrhizae transplantation from Panicum maximum as biofertilizer for paddy cultivation in Sri Lanka. Journal of Agriculture and Environment for International Development (JAEID), 118(2), 139–152. https://doi.org/10.36253/jaeid-16213

Copyright (c) 2024 Chathurika Wanninayake, Mekhala Chandrasekara, Sanath Hettiarachi, Wasantha Malaviarachchi, Lorenzo Vergani, Sara Borin

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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Abstract

Indiscriminate chemical fertilization in paddy fields is causing detrimental global impacts, therefore exploration of bio-based sustainable alternatives to compensate requirement of chemical fertilizers is necessary. This study was designed to evaluate use of Arbuscular Mycorrhizal Fungi (AMF) obtained from roots of a spontaneous weed grass (Panicum maximum), which demonstrated an average AMF root colonization of 75-85%, as self-produced biofertilizer for rice cultivation. Field trial was carried out in paddy field at Monaragala, Sri Lanka. Two traditional rice (Oriza sativa L.) varieties (Suwadel and Kuruluthuda) were selected, and Randomized Complete Block Design was employed with three replicates in eight different treatment plots per block.  Different chemical fertilizer regimes (100%, 50% and 25% of recommended fertilizer dose) and no amendments as controls were applied. AMF inocula were applied alone or supplemented to fertilized treatments (100% +AMF, 50% +AMF, 25% +AMF, AMF alone). Treatment plan was implemented separately for two varieties. Rice yield and percentage of AMF root colonization were quantified at harvest. Results revealed that grain dry weight for Suwadel was significantly higher (p<0.05) in treatments supplying AMF in addition with chemical fertilizer at optimal dose (100%) or 50% reduced, compared to other treatments. Highest number of seeds was observed in plants treated with 100% fertilizer, but result was not significantly different (p>0.05) from 50% +AMF and 100% + AMF treatments. Treatments with AMF inocula alone showed highest percentages of AMF colonization in Suwadel. Inverse connection was observed between fertilizer level and AMF colonization. Differently from Suwadel, no AMF colonization was observed in Kuruluthuda variety and no significant differences were observed in harvest for treatments with and without AMF application. According to results, usage of AMF inocula as on-field prepared biofertilizer combined with 50% of chemical fertilizer is effective in improving sustainable agriculture but efficacy of application strictly depends on crop variety.

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References

  1. Campo, S., Martín-Cardoso, H., Olivé., Pla, E., Catala-Forner, M., Martínez-Eixarch, M., & Segundo, B.S. (2020). Effect of Root Colonization by Arbuscular Mycorrhizal Fungi on Growth, Productivity and Blast Resistance in Rice. Rice, 42(13), https://doi.org/10.1186/s12284-020-00402-7
  2. Chen, K., Scheper, J., Fijen, T.P.M., & Kleijn, D. (2022). Potential tradeoffs between effects of arbuscular mycorrhizal fungi inoculation, soil organic matter content and fertilizer application in raspberry production. PLoS ONE, 18;17(7), e0269751. https://doi.org/10.1371/journal.pone.0269751
  3. Fall, A.F., Nakabonge, G., Ssekandi, J., Founoune-Mboup H, Badji A, Ndiaye A., … Ekwangu, J. (2023). Combined Effects of Indigenous Arbuscular Mycorrhizal Fungi (AMF) and NPK Fertilizer on Growth and Yields of Maize and Soil Nutrient Availability. Sustainability, 15(3), 1–12. https://doi.org/10.3390/su15032243
  4. Gajaweera, C., Weerasinghe, B., & Seresinhe, T. (2011). Preliminary study on the adaptation of wild Guinea grass (Panicum maximum) to different agro-climatic regions in Hambanthota District. In Proceedings of International Forestry and Environment Symposium (Vol. 16). https://doi.org/10.31357/fesympo.v16i0.70
  5. Gutierrez, A. & Grillo, M.A. (2022). Effects of Domestication on Plant–Microbiome Interactions, Plant and Cell Physiology, 63(11), 1654–1666, https://doi.org/10.1093/pcp/pcac108
  6. Hardy, A.G., Reddy, D.N., Venkatanarayana, M. & White, B.H. (2016). System of Rice Intensification provides environmental and economic gains but at the expense of social sustainability — A multidisciplinary analysis in India. Agricultural Systems, 143, 159-168. https://doi.org/10.1016/j.agsy.2015.12.012
  7. Ishaq, L., Adu-Tae, A.S.J., Airthur, M.A. & Bako, P.O. (2021). Effect of single and mixed inoculation of arbuscular mycorrhizal fungi and phosphorus fertilizer application on corn growth in calcareous soil. Biodiversitas Journal of Biological Diversity, 22(4), 1920–1926. https://doi.org/10.13057/biodiv/d220439
  8. Jiang, G., Zhang, Y., Gan, G., Li, W., Wan, W., Jiang, Y., … Dini-Andreote, F. (2022). Exploring rhizo-microbiome transplants as a tool for protective plant-microbiome manipulation. ISME Communications, 2(1),1–10. https://doi.org/10.1038/s43705-022-00094-8
  9. Juan, E., Pérez Jaramillo., Mendes, R. & Raaijmakers, J.M. (2016). Impact of plant domestication on rhizosphere microbiome assembly and functions. Plant molecular biology, 90,635–644. doi:10.1007/s11103-015-0337-7
  10. Kendzior, J., Warren-Raffa, D. & Bogdanski, A. (2022). The soil microbiome: a game changer for food and agriculture – Executive summary for policymakers and researchers. Rome, FAO. https://doi.org/10.4060/cc0717en
  11. Kuila, D. & Ghosh, S. (2022). Aspects, problems and utilization of Arbuscular Mycorrhizal (AM) application as biofertilizer in sustainable agriculture. Current Research in Microbial Sciences, 3, 100-107. https://doi.org/10.1016/j.crmicr.2022.100107
  12. McGonigle, T.P., Miller, M.H., Evans, D.G., Feirchild, G.L. & Swan, J.A. (1990). A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytologist, 115(03),495-501, https://doi.org/10.1111/j.1469-8137.1990.tb00476.x
  13. Mulyadi. & Jiang, L. (2023). Nitrogen fertilizer alters the physicochemical soil properties, nitrogen uptake, and rice yield in a polybag experiment. Agriculture, 13(7), 1364, https://doi.org/10.3390/agriculture13071364
  14. Naher, U.A., Othman, R., Ali Panhwar, Q. & Ismail, M.R. (2015). Biofertilizer for Sustainable Rice Production and Reduction of Environmental Pollution. In: Hakeem, K.R. (ed.) Crop Production and Global Environmental Issues. Springer, 283–291, https://doi.org/10.1007/978-3-319-23162-4
  15. Orozco-Mosqueda, M.D.C., Kumar, A., Babalola, O.O. & Santoyo, G. (2023). Rhizobiome Transplantation: A Novel Strategy beyond Single-Strain/Consortium Inoculation for Crop Improvement. Plants, 12, 3226, https:// doi.org/10.3390/plants12183226
  16. Naher, U.A., Ahmed, M.N., Imran, M., Sarkar, U., Biswas, J.C. & Panhwar, Q.A. (2018). Fertilizer management strategies for sustainable rice production. In: Organic Farming: Global Perspectives and Methods. Elsevier Inc, https://doi.org/10.1016/B978-0-12-813272-2.00009-4
  17. Panneerselvam, P., Kumar, U., Sugitha, T.C.K., Parameswaran, C., Sahoo, S., Binodh, A.K., Jahan, A. & Anandan, A. (2017). Arbuscular Mycorrhizal Fungi (AMF) for Sustainable Rice Production. Advances in Soil Microbiology: Recent Trends and Future Prospects: Soil-Microbe-Plant Interaction, 2,99-126, https://doi.org/10.1007/978-981-10-7380-9_6
  18. Qian, S., Xu, Y., Zhang, Y., Wang, X., Niu, X. & Wang, P. (2024). Effect of AMF inoculation on reducing excessive fertilizer use. Microorganisms, 12(8), 1550, https://doi.org/10.3390/microorganisms12081550
  19. Sandeepani, S.M.N.M., Weerakkody, W.J.S.K. & Karunarathne, K.H.M.I. (2018). Use of Panicum maximum as a Source of Bio-fertilizer and Biochar on Crop Response of Raphanus sativus L. in Organic Cultivation. Proceeding of International Research on Food Security, Natural Resource Management and Rural Development. https://www.tropentag.de/2018/abstracts/full/585.pdf
  20. Schader, C., Lampkin, N., Christie, M., Nemecek, T., Gaillard, G. & Stolze, M. (2013). Evaluation of cost-effectiveness of organic farming support as an agri-environmental measure at Swiss agricultural sector level. Land Use Policy. 31, 196-208, https://doi.org/10.1016/j.landusepol.2012.06.014
  21. Shruti-Kirti., Sharma, R., Bhawna-Dipta, R.P. & Kaushal, R. (2017). Mass multiplication of indigenous arbuscular multiplic mycorrhizae for temperate fruit crops. International quarterly journal of life science, 12(1), 145–148.
  22. Sirisena, D.N. (2013). Fertilizer recommendation for paddy cultivation. Department of Agriculture Peradeniya, Sri Lanka. Available online: https://doa.gov.lk/rrdi_fertilizerrecomendation_irrigated_izdz
  23. Řezáčová, V., Zemková, L., Beskid, O., Püschel, D., Konvalinková, T., Hujslová, M., Slavíková, R. & Jansa, J. (2018). Little cross-feeding of the mycorrhizal networks shared between C3-panicum bisulcatum and C4-panicum maximum under different temperature regimes. Frontiers in Plant Science: 1–16, https://doi.org/10.3389/fpls.2018.00449
  24. Rini, M.V., Yansyah, M.P. & Arif, M.A.S. (2021). The Application of Arbuscular Mycorrhizal Fungi Reduced the Required Dose of Compound Fertilizer for Oil Palm (Elaeis Guineensis Jacq.) in Nursery. IOP Conference Series: Earth and Environmental Science, 1012(1), https://doi.org/10.1088/1755-1315/1012/1/012011
  25. Trejo, D., Sangabriel-Conde, W., Gavito-Pardo, M.E. & Banuelos, J. (2021). Mycorrhizal inoculation and chemical fertilizer interactions in pineapple under field conditions. Agriculture (Switzerland), 11(10), 1–8. https://doi.org/10.3390/agriculture11100934
  26. Watanarojanaporn, N., Boonkerd, N., Tittabutr, P., Longtonglang, A., Young, J.P. & Teaumroong, N. (2013). Effect of rice cultivation systems on indigenous arbuscular mycorrhizal fungal community structure. Microbes and Environments, 28(3),316-24, doi:0.1264/jsme2.me13011
  27. Wang, L., Chen, X., Du, Y., Zhang, D. & Tang, Z. (2022). Nutrients Regulate the Effects of Arbuscular Mycorrhizal Fungi on the Growth and Reproduction of Cherry Tomato. Frontiers in Microbiology, 8(13),843010, doi:10.3389/fmicb.2022.843010
  28. Wang, Y., He, X. & Yu, F. (2022). Non-host plants: Are they mycorrhizal networks players. Plant Diversity, 44(2), 127–134. https://doi.org/10.1016/j.pld.2021.06.005
  29. Yang, F. Y., Li, G. Z., Zhang, D. E., Christie, P., Li, X. L., & Gai, J. P. (2010). Geographical and plant genotype effects on the formation of arbuscular mycorrhiza in Avena sativa and Avena nuda at different soil depths. Biology and Fertility of Soils, 46, 435-443.
  30. Zhang, H., Wang, L., Ma. F., Keith, J., Bloomfield., Yang, J., Owen, K. & Atkin. (2015). Is resource allocation and grain yield of rice altered by inoculation with arbuscular mycorrhizal fungi, Journal of Plant Ecology, 8(4), 436–448, https://doi.org/10.1093/jpe/rtu025